Fig. 5.

AA/RA medium supplementation in early canine reprogramming facilitates the upregulation of a subset of transcripts associated with pluripotency, proliferation, and LIF signaling. a Hierarchical cluster analysis to identify subsets of genes that show specific expression patterns across specific samples of early (6 DPI) canine reprogramming intermediates. Divergent color scale represents gene expression scaled by row. b Relative abundances of transcripts associated with cell surface proteins or the synthesis of cell surface antigens. c Relative abundances of transcripts associated with cell pluripotency. d Relative abundances of transcripts associated with early embryonic (naïve) pluripotency. e Relative abundances of transcripts associated with leukemia inhibitory factor (LIF)-signal transducer and activator of transcription 3 (STAT3) signaling. f Relative abundances of transcripts associated with cell proliferation. g Relative abundances of transcripts associated with transforming growth factor beta (TGFβ) signaling. Data are presented as mean ± standard error, n = 3. Means annotated with different letters are considered significantly different by one-way analysis of variance and Tukey’s honestly squared difference test, P < 0.05