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. Author manuscript; available in PMC: 2020 Dec 9.
Published in final edited form as: ACS Biomater Sci Eng. 2015 Oct 28;1(12):1220–1230. doi: 10.1021/acsbiomaterials.5b00445

Figure 1.

Figure 1.

(A) Osteogenic gene expression, (B) osteogenic protein expression, and (C) deposition by h-MSCs cultured on U-PCLDA and PD–PCLDA films for 2 weeks in the presence of osteogenic medium (OM) containing L-ascorbic acid-2-phosphate, β-glycerophosphate, and dexamethasone. Results were normalized to the U-PCLDA construct values. *, indicates a significant difference relative to day 0, p < 0.05. #, indicates a significant difference relative to U-PCLDA; p < 0.05. Note osteocalcin (OCN) was only examined at the mRNA level due to its low molecular weight, which prevented its analysis by Western blot using 8% or 10% SDS–PAGE gels and transfer times used herein.