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A. Representative images of scaffolds taken at indicated time points (green: B3T). Images are max projections of confocal z-stacks. (scale bar: 100 μm). B. Box and whisker plot of sum projections from 100-slice z-stacks to determine percent of the imaged area covered by B3T+ staining. (n=5 per time point). C. Example of 3D growth of neurons after 12 weeks of growth (scale bar: 100 μm)
