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. Author manuscript; available in PMC: 2020 Dec 9.
Published in final edited form as: Cell Rep. 2018 Oct 23;25(4):959–973.e6. doi: 10.1016/j.celrep.2018.09.077

Figure 7. OCN Signaling in the DG Upregulates RbAp48 and Ameliorates Age-Related Memory Loss.

Figure 7.

(A) Images of the DG from 16-month-old wild-type (WT) mice stained for RbAp48 (red) 48 hr after injection of PBS (20 ng) (top) or OCN (bottom) (scale bar, 100 μm).

(B) Quantification of RbAp48 protein after injection of OCN or PBS in the DG of 16-month-old WT mice. OCN increases RbAp48 levels (unpaired t tests, p = 0.0179; n = 4 mice per group).

(C) Images of the DG from 16-month-old WT mice stained for CBP (green) and BDNF (red), 48 hr after injection of PBS (20 ng) (top) or OCN (bottom) (scale bar, 100 μm).

(D and E) Quantification of CBP (D) and BDNF (E) protein levels after injection of OCN or PBS in the DG of 16-month-old WT mice. OCN increases CBP and BDNF levels (unpaired t tests, p < 0.0001 and p = 0.034, respectively; n = 6 mice per group).

(F) No differences were observed between the genotypes in total exploration time in the familiarization or the testing phase.

(G) Discrimination index for novel object after injections of PBS or OCN in the DG of 16-month-old WT mice (two-way RM ANOVA comparisons, post hoc Bonferroni, n = 16 mice per group treatment, p = 0.0036, Fam versus Nov, p = 0.0008). Aged mice injected with OCN in the DG were able to discriminate between the novel and familiar object.

(H and I) Contextual fear discrimination in 16-month-old mice injected with PBS (H) or OCN (I) in the DG/CA3c. Aged mice injected with OCN in the DG were able to discriminate between the two contexts (three-way RM ANOVA, post hoc Bonferroni for OCN or PBS and CTXA and CTXB, three-way RM ANOVA for treatment and context × treatment, p < 0.0001; n = 16 mice per group).

(J) Contextual fear discrimination at day 24 in 16-month-old mice injected with OCN or PBS. Aged mice injected with OCN in the DG were still able to discriminate between the two contexts (unpaired t test, p = 0.0395; n = 16 mice per group).

(K) Freezing levels of mice injected with PBS or OCN in CA3c 30 s after one 1.5-mA foot-shock.

(L) Freezing levels during 1–3 min of exposure to context D 48 hr after injection of OCN or PBS in CA3c (two-way RM ANOVA, post hoc Bonferroni, p = 0.04 for first minute; n = 16 mice per group).

All data represent means ± SEM. *p < 0.05, **p<0.01, ***p < 0.001.