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. 2020 Dec 9;15(12):e0243660. doi: 10.1371/journal.pone.0243660

Fig 6. Expression of PINK1, Parkin, and MFN2 after hindlimb unloading.

Fig 6

(A) Western blots of PINK1, Parkin, and MFN2 immunoreactivities. (B) Total membrane protein detected by SYPRO Ruby staining. (C-F) Comparisons of the immunoreactivities of PINK1, 50 kDa Parkin, approximately 40 kDa Parkin, and MFN2 expression normalized to total protein between groups are indicated in C, D, E, and F, respectively. (G) Comparison of relative MFN2 level calculated from each MFN2 band intensity normalized to total MFN2 is expressed in G as a percentage. CON, control. HU, hindlimb unloading. Data are expressed as means ± SE. **significantly different from CON (p < 0.01).