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. 2020 Nov 25;14(11):e0008308. doi: 10.1371/journal.pntd.0008308

Fig 2. Melt profile of field samples.

Fig 2

Three samples were identified as T. b. rhodesiense due to the production of both PLC and TBR peaks (samples:1027, 1079 and 1137). Samples which only produced PLC peaks were considered to be T. b. brucei (samples: 2362,2377 and 2380). The TBR positive control was T. b. rhodesiense DNA and the negative control used was nuclease free water. Normalized fluorescence dF/dt is plotted against degrees °C (deg.) with threshold indicated in red and species-specific bins shown in grey.