Table 1. HRM and PCR primers used for identification of single-copy gene targets of T. b. rhodesiense, T. b. gambiense and T. brucei s. l.
HRM primers were run in multiplex whilst PCR primers were run in singleplex. *This primer set was run in multiplex in HRM and in singleplex in PCR.
| Primer | Species | Primer sequence 5’-3’ | Product size (bp) | Product Tm (°C) | Reference | Assay |
|---|---|---|---|---|---|---|
| PLC1 | Trypanozoon | CAGTGTTGCGCTTAAATCCA | 319 | 79.1 | This study | HRM |
| PLC2 | CCCGCCAATACTGACATCTT | |||||
| TbRh1 | T. b. rhodesiense | GAAGCGGAAGCAAGAATGAC | 134 | 84.2 | This study | HRM |
| TbRh2 | GGCGCAAGACTTGTAAGAGC | |||||
| TgsGP1 | T. b. gambiense | GCTGCTGTGTTCGGAGAGC | 308 | 87.5 | [29] | HRM and PCR* |
| TGsGP2 | GCCATCGTGCTTGCCGCTC | |||||
| 657 | Trypanozoon | CGCTTTGTTGAGGAGCTGCAA GCA | 324 | - | [21] | PCR |
| 658 | TGCCACCGCAAAGTCGTTATT TCG | |||||
| SRA 02 | T. b. rhodesiense | AGCCAAAACCAGTGGGCA | 669 | - | [21] | PCR |
| SRA 03 | TAGCGCTGTCCTGTAGACGCT |