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. 2020 Dec 9;15(12):e0243644. doi: 10.1371/journal.pone.0243644

Fig 5. Peg13 mitigates the effect of sevoflurane by targeting miR-128-3p in NSCs.

Fig 5

A. FISH identified the cytoplasmic and nuclear distribution of Peg13 transcripts in NSCs. Magnification 100 x, Scale bar = 10 um. B. Quantitative RT-PCR revealed that sevoflurane exposure increased miR-128-3p transcripts in NSCs. C. Bioinformatics predicted the potential interaction between miR-128-3p with Peg13. D. Luciferase reporter assay indicated that miR-128-3p inhibited the Peg-13-regualted, but not its mutant-regulated, luciferase expression in 293T cells. E. Peg13 over-expression decreased miR-128-3p transcripts in NSCs. F. Transfection with different doses of miR-128-3p mimics induced miR-128-3p transcripts in NSCs in a dose-dependent manner, determined by qRT-PCR. G, H. Induction of miR-128-3p expression reduced neurosphere formation in the sevoflurane-exposed Peg13-over-expressed NSCs. J, K. Induction of miR-128-3p expression decreased EdU incorporation in the sevoflurane-exposed Peg13-over-expressed NSCs. L, O. Induction of miR-128-3p expression inhibited neuronal and oligodendrocyte differentiation of the sevoflurane-exposed Peg13-over-expressed NSCs. Data are representative images, charts or expressed as the mean ± SEM of each group of cells from three separate experiments. *P<0.05, **P<0.01, ***P <0.001, determined by Student T test.