Fig. 5. The inhibition of CDK-12 results in gradual premature termination of Pol II transcription within operons.

(A) Meta-operon profiles of BEADS-normalized Pol II occupancy data averaged over two replicates. The Pol II ChIP signal in the R (blue) and R + I (red) conditions is shown. The organization of the operons is shown at the bottom. Only the operons containing at least four genes were considered. Operons with any intergenic regions larger than 800 nt were also excluded from the analysis. (B) Meta-gene profiles of the median log₂ fold change between Pol II ChIP signal in the R and R + I conditions as in (A). (C) Meta-operon profiles of S2 phosphorylated Pol II occupancy normalized on total Pol II occupancy data averaged over two replicates. The organization of the operons is shown at the bottom.