Skip to main content
. 2020 Dec 9;6(50):eabc1450. doi: 10.1126/sciadv.abc1450

Fig. 6. CDK-12 is required for the efficient chromatin occupancy of the CstF complex.

Fig. 6

(A) Co-IP experiment of CTD S2P AMA-1 and CPF-2 (Csf64). Left: Nuclear extracts from fed L1 larvae were used for anti–CTD S2P IP and the precipitated material was separated on PAGE and submitted to anti–CTD S2P or anti–CPF-2 Western blotting (WB) as indicated. The ladder and corresponding size are shown. No IP refers to the crude nuclear extracts and beads only to IP where no antibodies were added. Middle: Same experiment performed on nuclear extracts from fed L1 larvae from the cdk-12-as strain. Right: Same experiment performed on nuclear extracts from fed L1 larvae from the cdk-12-as strain grown in the presence of the inhibitor. (B) ChIP experiments were performed on chromatin prepared from the indicated starvation (S), recovery (R), and recovery + inhibitor (R + I) conditions using anti–CTD S2P and anti–AMA-1 antibodies in triplicate. Amplicons marked from 1 to 12 allowed the scanning of the CEOP 3156 operon containing four genes. The CTD S2P signal was normalized on the total Pol II signal. (C) ChIP experiments were performed on chromatin prepared from the indicated starvation (S), recovery (R), and recovery + inhibitor (R + I) conditions using anti–CPF-1 (CstF50) antibodies in duplicate. Amplicons marked from 1 to 12 allowed the scanning of the CEOP 3156 operon containing four genes.