Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background D. melanogaster | Oregon-R | Flybase | ||
| Strain, strain background D. melanogaster | w; Df(2L)HisC FRT40A/Df(2L)HisCFRT40A; 12xHisGUwt/12 xHisGUwt | McKay et al., 2015 | ||
| Strain, strain background D. melanogaster | w; Df(2L)HisC FRT40A/CyO ubi-GFP; 12xHisGUH3K36R/TM6B | McKay et al., 2015 | ||
| Strain, strain background D. melanogaster | w; Df(2L)HisC FRT40A/CyO twi:Gal4 UAS:GFP; 3xHisGUH3K36A(VK33) 3xHisGUH3K36A(86Fb)/3xHisGUH3K36A(VK33) 3xHisGUH3K36A(86Fb) | This study | Available on request | |
| Strain, strain background D. melanogaster | w; Df(2L)HisC FRT40A/CyO twi:Gal4 UAS:GFP; 3xHisGUH3K36R(VK33) 3xHisGUH3K36R(86Fb)/3xHisGUH3K36R(VK33) 3xHisGUH3K36R(86Fb) | This study | Available on request | |
| Strain, strain background D. melanogaster | w; Df(2L)HisC FRT40A/CyO ubi:GFP; 3xHisGUH3K27R(68E) 3xHisGUH3K27R (86Fb)/3xHisGUH3K27R (68E) 3xHisGUH3K27R (86Fb) | Pengelly et al., 2013 | ||
| Strain, strain background D. melanogaster | w hs-flp; w; hs-nGFP FRT40A/hs nGFP FRT40; 3xHisGUH3K27R(68E) 3xHisGUH3K27R(86Fb)/3xHisGUH3K27R(68E) 3xHisGUH3K27R (86Fb) | Pengelly et al., 2013 | ||
| Strain, strain background D. melanogaster | w hs-flp; M(2)25A ubi-GFP FRT40A/CyO | Müller lab stocks | Available on request | |
| Strain, strain background D. melanogaster | yw; esc6 b pr/CyO, P[esc+] | Struhl laboratory Struhl, 1981 |
||
| Strain, strain background D. melanogaster | In(2LR) Gla/CyO, esc2 | Struhl laboratory Struhl, 1981 |
||
| Strain, strain background D. melanogaster | w hs-flp; hs-nGFP FRT2A/hs nGFP FRT2A | Beuchle et al., 2001 | ||
| Strain, strain background (Escherichia coli) | BL21(DE3) | Sigma-Aldrich | CMC0016 | Electrocompetent cells |
| Cell line (Trichoplusia ni) | HighFive cell line for expression | Invitrogen | Product nr.: B85502 BTI-Tn-5B1-4 (RRID:CVCL_C190) |
Protein expression |
| Cell line (Spodoptera frugiperda) | Sf21 cell line for Baculovirus production | Invitrogen | Product nr.: 1149701 (RRID:CVCL_0518) | Baculovirus production for protein expression |
| Antibody | H3K27me3 Rabbit monoclonal antibody |
Cell Signaling Technology |
Cell Signaling Technology #9733 | IF (1:50) WB (1:2000) ChIP (1:500) |
| Antibody | H3K27me3 Rabbit polyclonal antibody |
Millipore | Millipore #07–449 |
WB (1:1000) |
| Antibody | H3K27me1 Rabbit polyclonal antibody |
Millipore | Millipore #07–448 |
WB (1:6000) |
| Antibody | H3K36me3 Rabbit monoclonal antibody |
Cell Signaling Technology |
Cell Signaling Technology #4909 | WB (1:750) |
| Antibody | H3K36me2 Rabbit monoclonal antibody |
Cell Signaling Technology |
Cell Signaling Technology #2901 | WB (1:250) |
| Antibody | H3K36me2 Rabbit monoclonal antibody |
Abcam | #9049 | ChIP (1:300) |
| Antibody | H2B Rabbit polyclonal antibody |
This study | Raised against full-length recombinant Drosophila H2B | WB (1:10000) Available on request |
| Antibody | H4 Rabbit polyclonal antibody |
Abcam | Abcam #10158 |
WB (1:200000) |
| Antibody | Caf1 Rabbit polyclonal antibody |
Gambetta et al., 2009 | Müller lab | WB (1:10000) |
| Antibody | Abd-B Mouse monoclonal antibody |
DHSB | DSHB (1A2E9) | IF (1:300) |
| Antibody | Antp Mouse monoclonal antibody |
DHSB | DSHB (8C11) | IF (1: 100) |
| Recombinant DNA reagent | pfC31-attB-3xHisGU.H3K36A | This study | See Materials and Methods Available on request |
|
| Recombinant DNA reagent | pfC31-attB-3xHisGU.H3K36R | This study | See Materials and Methods Available on request |
|
| Recombinant DNA reagent | nucleosome-positioning sequence 601 (147 bp + linker version) |
Lowary and Widom, 1998, Nekrasov et al., 2005 | ||
| Recombinant DNA reagent | pFB-EZH2 | Choi et al., 2017 | N-terminal 6xHis-tag | |
| Recombinant DNA reagent | pFB-EZH2CXC>A | This study | N-terminal 6xHis-tag See Materials and Methods Available on request |
|
| Recombinant DNA reagent | pFB-EED | Choi et al., 2017 | N-terminal 6xHis-tag | |
| Recombinant DNA reagent | pFB-EEDEED>A | This study | N-terminal 6xHis-tag See Materials and Methods Available on request |
|
| Recombinant DNA reagent | pFB-SUZ12 | Choi et al., 2017 | N-terminal 6xHis-tag | |
| Recombinant DNA reagent | pFB-RBBP4 | Choi et al., 2017 | N-terminal 6xHis-tag | |
| Recombinant DNA reagent | pFB-PHF1 | Choi et al., 2017 | N-terminal twin-strep and 6xHis-tag (SHT) | |
| Recombinant DNA reagent | pFB-PHF1C
(PHF1515-567) |
Choi et al., 2017 | N-terminal twin-strep and 6xHis-tag (SHT) | |
| Peptide, recombinant protein | H318-42 peptide | MPIB core facilty | ||
| Peptide, recombinant protein | H318-42K36me3 peptide | MPIB core facilty |