Skip to main content
. 2020 Nov 25;9:e59489. doi: 10.7554/eLife.59489

Figure 2. Wnt3 source and target regions at 24 hpf.

3D dorsal projection of Wnt3 (A) source regions at 24 hpf and (B) target regions at 24 hpf (top view). 3D ventral projection of Wnt3 (C) source regions at 24 hpf and (D) target regions at 24 hpf (bottom view). (E) 3D projection of Wnt3 source and target regions at 24 hpf (lateral view). See Video 3 for a detailed view. dCe, dorsal regions of cerebellum; Epi, epithalamus; MHB, midbrain–hindbrain boundary; OT, optic tectum; vCe, ventral regions of cerebellum. Images orientation: anterior to the left. Scale bar 30 μm.

Figure 2.

Figure 2—figure supplement 1. Fluorescence correlation spectroscopy (FCS) measurements of Wnt3EGFP and PMTmApple in the Wnt3 target regions at 24 hpf.

Figure 2—figure supplement 1.

Expression of (A) PMTmApple and (B) Wnt3EGFP in Tg(−4.0wnt3:Wnt3EGFP) × Tg(−4.0wnt3:PMTmApple) double transgenic embryos at 24 hpf. Representative autocorrelation function (ACF) for (C) PMTmApple and (D) Wnt3EGFP in the OT at 24 hpf. No ACFs were obtained for PMTmAPple in the target regions indicating an absence of mApple signal in these regions. Ce, cerebellum; MHB; midbrain–hindbrain boundary; OT, optic tectum. Images orientation: anterior to the left. Scale bar 30 μm.
Figure 2—figure supplement 2. Expression of EGFP in Tg(−4.0wnt3:EGFP) at 24 hpf.

Figure 2—figure supplement 2.

The expression of EGFP in Tg(-4.0wnt3:EGFP) closely mimics the expression of PMTmApple in Tg(−4.0wnt3:PMTmApple). Ce, cerebellum; MHB; midbrain–hindbrain boundary. Images orientation: anterior to the left. Scale bar 30 μm.
Figure 2—figure supplement 3. Expression of wnt3 transcripts and downstream wnt signaling transcription factor at 24 hpf.

Figure 2—figure supplement 3.

(A) Whole mount in situ hybridization (dorsal view) of wnt3 at 24 hpf. The expression pattern is similar to previously published results in Clements et al., 2009, Duncan et al., 2015, and Teh et al., 2015. (B) Expression of NLS-mCherry in wnt reporter line Tg(7XTcf-Xla.Siam:NLS-Cherry) at 24 hpf. dCB, cerebellum; Epi, epithalamus; FP, floor plate; MHB, midbrain–hindbrain boundary; mRP, midbrain roof plate; OT, optic tectum. Orientation: anterior to left.