Table 1. Influence of heparan sulfate proteoglycans on the dynamics of Wnt3EGFP, LynEGFP, and secretedEGFP at 48 hpf. Data are mean ± SD.
Table 1—source data 1. Tg(−4.0wnt3:Wnt3EGFP) embryos treated by heparinase and surfen.
(A) The expression of Wnt3EGFP after heparinase treatment. (B) Distribution of Dextran-TRITC coinjected with heparinase in the BV of Tg(−4.0wnt3:Wnt3EGFP) embryo. (C) Expression of Wnt3EGFP after surfen treatment. BV, brain ventricle; Ce, cerebellum. Images orientation: anterior to the left. Scale bar 50 μm.
elife-59489-table1-data1.pdf (1.3MB, pdf)
Table 1—source data 2. Individual fluorescence correlation spectroscopy measurements of Wnt3EGFP, LynEGFP, and secEGFP embryos before and after heparan sulfate proteoglycan disruption.
elife-59489-table1-data2.xlsx (44.7KB, xlsx)
| Sample | Dfast (µm2/s) | Dslow (µm2/s) | Fslow | No. of measurements |
|---|---|---|---|---|
| Wnt3EGFP | 24.7 ± 4.8 | 0.6 ± 0.3 | 0.6 ± 0.02 | 47 |
| Wnt3EGFP + heparinase | 43.4 ± 7.6 | 0.4 ± 0.2 | 0.6 ± 0.04 | 63 |
| Wnt3EGFP + surfen | 42.0 ± 6.9 | 0.5 ± 0.3 | 0.6 ± 0.08 | 30 |
| LynEGFP | 39.1 ± 11.2 | 2.2 ± 0.6 | 0.7 ± 0.04 | 29 |
| LynEGFP + heparinase | 40.1 ± 9.5 | 2.7 ± 0.7 | 0.6 ± 0.04 | 35 |
| LynEGFP + surfen | 41.4 ± 6.9 | 3.1 ± 0.9 | 0.7 ± 0.06 | 18 |
| SecEGFP | 59.4 ± 9.4 | - | - | 30 |
| SecEGFP + heparinase | 56.9 ± 9.7 | - | - | 30 |
| SecEGFP + surfen | 53.5 ± 8.5 | - | - | 18 |