REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
RNF12	Novus Biologicals	Cat#H00051132-M01; RRID: AB_547742
ERK1	BD Biosciences	Cat#610408; RRID: AB_397790
SRPK1	BD Biosciences	Cat#611072; RRID: AB_398385
SRPK2	BD Biosciences	Cat#611118; RRID: AB_398429
HA-tag	Abcam	Cat#ab9110; RRID: AB_307019
REX1	Abcam	Cat#ab28141; RRID: AB_882332
SRPK3	R&D Systems	Cat#MAB7230-SP
FLAG	Sigma Aldrich	Cat#F1804-50UG; RRID: AB_262044
HA-HRP	Roche	Cat#12013819001; RRID: AB_390917
Synaptophysin	Cell Signaling Technologies	Cat#5461 (D35E4); RRID: AB_10698743
beta-actin	Cell Signaling Technologies	Cat#4970 (13E5); RRID: AB_2223172
RNF12 (1-271)	MRC-PPU Reagents and Services	Cat#S691D third bleed
RNF12 pSer212/214 (QRRARpSRpSPEHRR)	MRC-PPU Reagents and Services	Cat#SA310 fourth bleed
GST	MRC-PPU Reagents and Services	Cat#S902A third bleed
Phosphoepitope SR proteins	Millipore	Cat#MABE50 clone 1H4; RRID: AB_10807429
KLF4	R&D Systems	Cat#AF3158; RRID: AB_2130245
MAP2	Sigma Aldrich	Cat#M2320; RRID: AB_609904
HA-tag	Sigma Aldrich	Cat#A2095 agarose beads; RRID: AB_257974
Chemicals, Peptides, and Recombinant Proteins
AZ191	Tocris	Cat#5232
KH-CB19	Merck Millipore	Cat#219511
CLK-IN-T3	Aobious	Cat#AOB8827
SPHINX31	Axon Medchem	Cat#Axon 2714
CHIR99021	Axon Medchem	Cat#Axon 1386
PD0325901	Axon Medchem	Cat#Axon 1408
VX-745	Selleckchem	Cat#S1458
JNK-IN-8	Selleckchem	Cat#S4901
RO-3306	Sigma Aldrich	Cat#SML0569
Flavopiridol	Stratech	Cat#S2679
CCT241533	Cayman	Cat#CAY19178
Harmine	Sigma Aldrich	Cat#286044
WEHI-345	Cayman	Cat#CAY23023
IRAK-4 Ina	MRC-PPU Reagents and Services	N/A
GSK461364	Cayman	Cat#CAY18099
SRPIN340	Sigma Aldrich	Cat#SML1088
MG132	Sigma Aldrich	Cat#C2211
Cycloheximide	Sigma Aldrich	Cat#C7698
Leptomycin B	Sigma Aldrich	Cat#L2913
Madrasin (DDD00107587)	Kind gift of Dr. Andrea Pawellek (University of Dundee)	N/A
Phos-Tag	MRC-PPU Reagents and Services	N/A
RNF12	MRC-PPU Reagents and Services	DU61098
RNF12 S212A S214A S227A S229A	MRC-PPU Reagents and Services	DU53249
DYRK1a	MRC-PPU Reagents and Services	DU19040
CLK2	MRC-PPU Reagents and Services	DU16987
GSK3beta	MRC-PPU Reagents and Services	DU899
ERK1 (MAPK3)	MRC-PPU Reagents and Services	DU1509
ERK2 (MAPK1)	MRC-PPU Reagents and Services	DU650
JNK3 alpha 1 (SAPK1b)	MRC-PPU Reagents and Services	DU1511
p38 alpha (SAPK2a)	MRC-PPU Reagents and Services	DU979
CDK2 - CyclinA	MRC-PPU Reagents and Services	DU43557
CDK5 - p35	MRC-PPU Reagents and Services	DU39816
CDK7 - MAT1 - Cyclin H	MRC-PPU Reagents and Services	DU49574
CDK9 - Cyclin T1	MRC-PPU Reagents and Services	DU31050
REX1	MRC-PPU Reagents and Services	DU53244
SMAD7	MRC-PPU Reagents and Services	DU19219
SRPK1	MRC-PPU Reagents and Services	DU967
SRPK2	MRC-PPU Reagents and Services	DU36135
SRPK3	MRC-PPU Reagents and Services	DU967
SRPK1 D497A	MRC-PPU Reagents and Services	DU66208
SRPK2 D541A	MRC-PPU Reagents and Services	DU66209
SRPK3 H159D	MRC-PPU Reagents and Services	DU61121
SRPK3 T211M	MRC-PPU Reagents and Services	DU61140
SRPK3 K270M	MRC-PPU Reagents and Services	DU61135
Ube1	MRC-PPU Reagents and Services	DU32888
UBE2D1 (UbcH5a)	MRC-PPU Reagents and Services	DU4315
FLAG-Ubiquitin	MRC-PPU Reagents and Services	DU46789
Ubiquitin	MRC-PPU Reagents and Services	DU20027
Ubiquitin IR-800	Walden lab (Uni of Glasgow)	N/A
Critical Commercial Assays
SRPKIN-1 kinase inhibitor profiling	This paper	http://www.kinase-screen.mrc.ac.uk/services/express-screen
Deposited Data
Raw RNA-SEQ data	This paper	GEO: GSE149554
Raw Brain Cortex Single Nucleus RNA-SEQ data	Hodge et al., 2019	dbGAP: phs001790
Raw hiPSC mass-spectrometry data	Brenes et al., 2020	PRIDE: PXD010557
Experimental Models: Cell Lines
Mouse: Embryonic Stem Cells	Laboratory of Janet Rossant, SickKids Research Institute, Toronto	CCE line
Human: induced Pluripotent Stem Cells	Cellartis AB Human Pluripotent Stem Cell Core Facility, School of Life Sciences, University of Dundee	CHiPS4 line
Human: Neuro2A	ATCC	Cat#CCL-131
Human: HEK 293	ATCC	Cat#CRL-1573
Human: U2OS	ATCC	Cat#HTB-96
Human: MCF7	ATCC	Cat#CRL-3435
Experimental Models: Organisms/Strains
Mouse: C57B6/J	Animal Facility, School of Life Sciences, University of Dundee	N/A
Oligonucleotides
Primers for qRT-PCR, see Table S8	This paper	N/A
Primers for genomic DNA sequencing, see Table S8	This paper	N/A
gRNA sequences for CRISPR/Cas9, see Table S8	This paper	N/A
ON-TARGETplus Srpk2 siRNA 06	Horizon Discovery	Cat#J-055142-06-0010
Non-targeting Pool siRNA	Horizon Discovery	Cat#D-001810-10-05
Recombinant DNA
pCAGGS PURO RNF12	MRC-PPU Reagents and Services	DU50610
pCAGGS PURO RNF12 S212A	MRC-PPU Reagents and Services	DU53528
pCAGGS PURO RNF12 S214A	MRC-PPU Reagents and Services	DU50796
pCAGGS PURO RNF12 S227A	MRC-PPU Reagents and Services	DU53591
pCAGGS PURO RNF12 S229A	MRC-PPU Reagents and Services	DU53592
pCAGGS PURO RNF12 S212A S214A	MRC-PPU Reagents and Services	DU53518
pCAGGS PURO RNF12 S227A S229A	MRC-PPU Reagents and Services	DU53514
pCAGGS PURO RNF12 S214A S229A	MRC-PPU Reagents and Services	DU53593
pCAGGS PURO RNF12 S212A S214A S227A S229A	MRC-PPU Reagents and Services	DU50797
pCAGGS PURO RNF12 delta SR-motif	MRC-PPU Reagents and Services	DU53413
pCAGGS PURO HA-RNF12	MRC-PPU Reagents and Services	DU50854
pCAGGS PURO HA-RNF12 S212A S214A S227A S229A	MRC-PPU Reagents and Services	DU58741
pCAGGS PURO RNF12 W576Y	MRC-PPU Reagents and Services	DU50800
pCAGGS PURO FLAG SRPK1	MRC-PPU Reagents and Services	DU53820
pCAGGS PURO FLAG SRPK2	MRC-PPU Reagents and Services	DU53821
pKN7 RLIM ex5 KO Sense A	MRC-PPU Reagents and Services	DU52037
pX335 RLIM ex5 KO antisense A + Cas9n	MRC-PPU Reagents and Services	DU52046
pBabeD P U6 RLIM (mouse) Cter KI Sense A	MRC-PPU Reagents and Services	DU57881
pX335 RLIM (mouse) Cter KI AntiSense A	MRC-PPU Reagents and Services	DU57891
pMA RLIM Cter R575C IRES-GFP donor	MRC-PPU Reagents and Services	DU57963
pMA RLIM Cter del 206-229 IRES-GFP donor	MRC-PPU Reagents and Services	DU57964
pMA RLIM Cter S212A S214A S227A S229A IRES-GFP donor	MRC-PPU Reagents and Services	DU57966
pMA RLIM Cter wt control IRES-GFP donor	MRC-PPU Reagents and Services	DU57967
pMA RLIM Cter W576Y IRES-GFP donor	MRC-PPU Reagents and Services	DU60290
pBabeD P U6 Srpk1 (mouse) ex3 KO Sense B	MRC-PPU Reagents and Services	DU60949
pX335 Srpk1 (mouse) ex3 KO Antisense B	MRC-PPU Reagents and Services	DU64462
pBabeD P U6 SRPK2 (mouse) ex5 KO Sense A	MRC-PPU Reagents and Services	DU64247
pX335 SRPK2 (mouse) ex5 KO Antisense A	MRC-PPU Reagents and Services	DU 64251
pBabeD P U6 ZFP42 (mouse) ex4 KO Sense A	MRC-PPU Reagents and Services	DU60065
pX335 ZFP42 (mouse) ex4 KO AntiSense A	MRC-PPU Reagents and Services	DU60072
Software and Algorithms
ScanProsite	Hulo et al., 2006	https://prosite.expasy.org/scanprosite/
Image Studio	LICOR Biosciences	https://www.licor.com/bio/image-studio/
Image Lab	Bio-Rad	https://www.bio-rad.com/en-uk/product/image-lab-software?ID=KRE6P5E8Z
Kinoviewer	Brenes and Lamond, 2019	https://peptracker.com
Proteome Discoverer v.2.0	Thermofisher	https://www.thermofisher.com/order/catalog/product/OPTON-30812?SID=srch-srp-OPTON-30812#/OPTON-30812?SID=srch-srp-OPTON-30812
Mascot	Matrix Science	https://www.matrixscience.com/server.html
Image J	NIH	https://imagej.nih.gov/ij/download.html
STAR software (v2.7.1a)	Dobin et al., 2013	https://github.com/alexdobin/STAR
HTSeq (v0.11.2)	Anders et al., 2015	https://htseq.readthedocs.io/en/master/
SARTools (v1.6.9)	Varet et al., 2016	https://github.com/PF2-pasteur-fr/SARTools
DESeq2 (v1.24)	Love et al., 2014	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
GOstats (v2.50.0)	Falcon and Gentleman, 2007	https://www.bioconductor.org/packages/release/bioc/html/GOstats.html
GraphPad Prism (v7.0c)	GraphPad Software Inc.	https://www.graphpad.com/scientific-software/prism/