A. muciniphila administration tightens the gut barrier and reduces systemic inflammation in the femur fracture mouse model. (A-E) qRT-PCR measurements of colonic mRNA levels of Cldn-2, -3 and -15, as well as of Ocln and Jam3 at 2 and 6 WPF. For 2 weeks, n=6 per group; for 6 weeks, n=7 per group. (F) Serum FITC-dextran levels were measured 4 h after FITC-dextran oral administration at 2 and 6 WPF. n=5 per group. (G) LPS levels in serum samples at 2 and 6 WPF. n=8 per group. (H-J) qRT-PCR analyses of colonic mRNA levels of Tnf-α, Il17A and Il10 at 2 and 6 WPF. For 2 weeks, n=6 per group; for 6 weeks, n=7 per group. (K-M) Serum concentrations of TNF-α (K), IL10 (L) and SAA3 (M) detected by ELISA at 1, 2, 4 and 6 WPF. n=5 per group. Data are mean+s.d. Whiskers represent minimum and maximum values, and boxes represent the interquartile range. *P<0.05, **P<0.01, unpaired two-tailed Student's t-test (A-J) or two-way ANOVA with Bonferroni post-hoc test (K-M).