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. 2020 May 13;27(12):960–975. doi: 10.1038/s41417-020-0178-7

Fig. 6. Targeted deep-sequencing of SaCas9.

Fig. 6

a, b Numbers of sequencing reads for the on-target (OnT) and off-target (OT) sites in C2C12 cells. c Targeted deep-sequencing to measure the muscle tissues of the OT sites. The mismatches of each OT site with the OnT protospacers is highlighted (red).