Fig. 2.

Phylogenetic relationships of known koala MHC gene alleles from class I (a) and class II (b). These maximum likelihood phylogenetic trees were generated using DNA sequences aligned with mafft (Katoh et al. 2002) before ModelFinder determined the best fit model (HKY + F + G4 for (a); TIMe + G4 for (b) (Kalyaanamoorthy et al. 2017)) and IQ-TREE (Nguyen et al. 2015) and UFBoot2 (Hoang et al. 2018) constructed the tree with 1000 bootstrap replicates. Only bootstrap values above 70 are shown. Alleles highlighted in blue were detected in this study. The accession number for each allele is presented in parenthesises after the allele name