. 2020 Oct 20;72(9):499–506. doi: 10.1007/s00251-020-01181-7

Table 1.

PCR primers used to identify MHC alleles

MHC class	Gene target	Primer sequence^a	Amplicon size (bp)	Reference
Class I	UA	MHCI_UA_F: ACCCCTGACCCTGCCGTGTC	313	Cheng et al. (2018)
	UA	MHCI_UA_R: CACCGCCTTCGCTCTGGTTGA	313	Cheng et al. (2018)
	UC	MHCI_UC_F: AAGGTCTCCAATGTTTCCGACTCA	397	Cheng et al. (2018)
	UC	MHCI_UC_R: TCTCGCGCTAAGGCCATACC	397	Cheng et al. (2018)
Class II	DAb	MHCII_DAb_F: ATGCCCCAAAGCACTTCAC	271	Lau et al. (2013)
	DAb	MHCII_DAb_R: CGCACTRAGAAGGGCTCA	271	Lau et al. (2013)
	DBb	MHCII_DBb_F: AGGGACATCCCAGAGGATTTCG	282	Lau et al. (2013)
	DBb	MHCII_DBb_R: TCTTCTGTCCACCGCGAAGG	282	Lau et al. (2013)
	DCb	MHCII_DCb_F: GGTGAGGTCTGAGTGTCACA	200	Abts et al. (2018)
	DCb	MHCII_DCb_R: CATTCACTATGGACCTTGCAGT	200	Abts et al. (2018)
	DMb	MHCII_DMb_F: CATGTGGAGAGTGGCTGTATG	266	Abts et al. (2018)
	DMb	MHCII_DMb_R: GTCCTTTGGGTCAACGCTC	266	Abts et al. (2018)

^aFor Illumina MiSeq sequencing, adaptor sequences were added to the 5′ ends of each primer to allow for multiplex barcoding: forward adaptor: TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG; reverse adaptor: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG