Figure 5.

BAFF blockade increases the protein levels of ZO-1 and occludin via NF-κB/MLCK/MLC signaling. Mice were euthanatized and samples were collected 4 h after LPS challenge. The expression of proteins was determined by western blot (n=3 for each group). Representative blots were shown and densitometric quantification data were expressed as the intensity ratio of target protein to GAPDH or corresponding controls. (A) ZO-1; (B) occludin; (C) p-MLC/MLC; (D) MLCK; (E) p-p65/p65; (F) p-IκB-α/IκB-α. *and** indicate P<0.05 and P<0.01, respectively compared with control group; ^# and ^## indicate P<0.05 and P<0.01, respectively compared with LPS group.