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. 2020 Dec 9;10:21565. doi: 10.1038/s41598-020-78669-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profiles in S. mansoni females of selected lincRNAs differentially expressed after 5-AzaC treatment (491 µM). Eight lincRNAs were selected after re-analysis of RNA-Seq public datasets of 5-AzaC treated S. mansoni females¹⁶ for validation by RT-qPCR in females. For each of the eight selected lincRNAs, the expression profiles obtained with RNA-Seq are shown on the left as TPM (transcripts per million), whereas the RT-qPCR results are shown on the right: (A) SmLINC133371-IBu; (B) SmLINC151825-IBu; (C) SmLINC158444-IBu; (D) SmLINC110084-IBu; (E) SmLINC158969-IBu; (F) SmLINC156349-IBu; (G) SmLINC103888-IBu; (H) SmLINC100882-IBu. For the RNA-Seq data, three biological replicates were analyzed; the fold-changes and p values represented by asterisks that are shown in the brackets were obtained using DESeq2. For the RT-qPCR data, mean ± SEM from five biological replicates are shown, and Student unpaired two-sided t test was applied. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.