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. 2020 Dec 9;10:21565. doi: 10.1038/s41598-020-78669-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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RNA-seq expression profiles at different S. mansoni stages of selected lincRNAs differentially expressed after 5-AzaC treatment (491 µM). The expression levels (shown as log2 of normalized counts) of the eight lincRNAs whose gene IDs are indicated at the top of each panel are shown. These lincRNAs were selected after re-analysis of RNA-Seq public datasets of 5-AzaC treated S. mansoni females¹⁶. The y-axis shows the expression level for each lincRNA in the RNA-seq assays (log2 of normalized counts) as determined at the stage indicated in the x-axis as follows: miracidia/sporocysts (M/S), cercariae (C), schistosomula (S), juveniles (J), adult males (M), adult females (F), posterior somatic tissues (P), heads (H) and tails (T). (A) SmLINC133371-IBu; (B) SmLINC151825-IBu; (C) SmLINC158444-IBu; (D) SmLINC110084-IBu; (E) SmLINC158969-IBu; (F) SmLINC156349-IBu; (G) SmLINC103888-IBu; (H) SmLINC100882-IBu. Only transcripts that were upregulated in one stage/tissue when compared with all others were considered as significantly more expressed in that stage/tissue and are marked with an asterisk. *p value < 0.05.