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. 2020 Dec 9;10:21583. doi: 10.1038/s41598-020-78395-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Viability, population doubling time, growth rate, and total cell yield of cultured human nasal septal chondrocytes (hNSCs). (a) The mean percentage of viable GF-treated hNSCs based on the trypan blue exclusion test. (b) Population doubling time was significantly lower in GF-treated hNSCs compared with the control medium of 2%HS starting from P1 to P6. (c) The growth rate (cells/cm²/day) was significantly higher in GF-treated hNSCs compared with the control medium of 2%HS starting from P1 to P6. (d) Total cumulative cell yield of GF-treated hNSCs and the control medium 2%HS were 3.04 × 10¹⁰ vs. 2.73 × 10⁸ cells respectively. P—passage number. GF—Growth Factors. (*) represents a statistically significant difference when p < 0.05 as analysed by one-way ANOVA. Values are presented as mean ± SEM, (n = 6).