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. 2020 Dec 9;10:21583. doi: 10.1038/s41598-020-78395-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Genotoxicity testing of Growth factor cocktail medium by comet assay. (a,b) Analysis of DNA damage by comet assay measuring tail moment which is the percentage of DNA in the tail and the mean interval of migration in the tail. Olive moment is the outcome of the proportion of total DNA in the tail and the distance between the centres of head mass and tail area. No significant difference was detected between all groups, p > 0.05, as analysed by two-tailed unpaired Student’s t-test. (d) A representative picture of DNA of hNSCs cultured in GFs with an insignificant level of DNA damage. (c) A representative picture of damaged DNA of chondrocytes treated with 100 μmol/L hydrogen peroxide. GF—Growth Factors Cultured Chondrocytes. P—passage number; C—Control. Measurement was presented as mean ± SEM, (n = 6).