Table 1.

Primers of genes analysed in RT-qPCR.

Gene	Accession no	Gene description	Primer sequence	PCR product size (bp)
GAPDH	NM_002046	Human glyceraldehyde 3-phosphate dehydrogenase	F: 5′-tcc ctg agc tga acg gga ag-3’ R: 5′-gga gga gtg ggt gtc gct gt-3’	217
P21	NM_002577.3	Human cyclin-dependent kinase inhibitor 1	F: 5′-gatggcaccagaggtggtta-3’ R: 5′-tcccgaaatattggggaaag-3’	198
P53	NM_000546.3	Human tumour suppressor gene	F: 5′-ggaagagaatctccgcaagaa-3’ R: 5′-agctctcggaacatctcgaag-3’	177
RB	NM_000321	Human retinoblastoma gene	F: 5′-cagacccagaagccattgaa-3’ R: 5′-ctgggtgctcagacagaagg-3’	115

(1) GAPDH: Human Glyceraldehyde 3-phosphate dehydrogenase as a reference gene. (2) P21: Human Cyclin-Dependent Kinase Inhibitor 1. (3) TP53: Human Tumour Suppressor gene. (4) PRB: human retinoblastoma gene. The primer sequences were designed using a wild-type TP53 sequence provided by the GenBank—the NIH genetic sequence database.