Table 2.

Sequences of primer pairs used for amplification of target and reference genes.

Gene	Primer sequence	Size	Acc (reference)
Claudin-1	GTGTTCAGAGGCATCAGGTATC GTCAGGTCAAACAGAGGTACAA	107	NM_001013611.2
Claudin-3	CCCGTCCCGTTGTTGTTTTG CCCCTTCAACCTTCCCGAAA	126	NM_204202.1 (28)
Occludin	CCGTAACCCCGAGTTGGAT ATTGAGGCGGTCGTTGATG	214	NM_205128.1
Zonula occluden-1	GGAGTACGAGCAGTCAACATAC GAGGCGCACGATCTTCATAA	101	XM_413773
Zonula occluden-2	GCGTCCCATCCTGAGAAATAC CTTGTTCACTCCCTTCCTCTTC	89	NM_204918
Interferon-γ	GCTCCCGATGAACGACTTGA TGTAAGATGCTGAAGAGTTCATTCG	63	NM_205149.1
Interleukin-1β	CCCGCCTTCCGCTACA CACGAAGCACTTCTGGTTGATG	66	XM_015297469.1
Interleukin-10	CGCTGTCACCGCTTCTTCA CGTCTCCTTGATCTGCTTGATG	63	NM_001004414.2
Interleukin-17A	AGCTGGACCACAGCGTCAAC GGCGGAGGACGAGGATCT	57	NM_204460.1
sIgAa	GTCACCGTCACCTGGACTACA ACCGATGGTCTCCTTCACATC	192	S40610.1
PGC-1αb	ACGGAGTTCCAATCGC AACCCTTACAACCTTCACAA	220	NM_001001464.1
mTORc	CATGTCAGGCACTGTGTCTATTCTC CTTTCGCCCTTGTTTCTTCACT	77	XM_417614.6
AMPK-α1d	ATCTGTCTCGCCCTCATCCT CCACTTCGCTCTTCTTACACCTT	125	NM_001039603
GAPDHe	CCTAGGATACACAGAGGACCAGGTT GGTGGAGGAATGGCTGTCA	64	NM_204305

For each gene, the primer sequence for forward (F) and reverse (R) (5′-3′) primers, the amplicon size (bp) and the NCBI Accession number (Acc) used for the primer design are listed.

^aSecretory immunoglobulin A.

^bPeroxisome proliferator-activated receptor-γ coactivator 1α.

^cMechanistic target of rapamycin.

^dAMP-activated protein kinase-α1.

^eGlyceraldehyde 3-phosphate dehydrogenase.