Table 6.
Comparison of microbial population in broiler chickens fed various dietary supplements on d 8 and 42 using 16S rRNA sequencing.
| Dietary treatmentsb | ||||
|---|---|---|---|---|
| Relative abundance (%) | NC | PC | PROB | PPEO |
| d 8 | ||||
| Actinobacteria | 1.95 | 2.72 | 0.93 | 0.54 |
| Bacteroidetes | 14.58 | 14.60 | 3.57 | 1.32 |
| Epsilonbacteraeota | 0.08 | 0.02 | 0.01 | 0.00 |
| Firmicutes | 81.82 | 76.35 | 94.21 | 97.67 |
| Proteobacteria | 1.39 | 5.99 | 0.95 | 0.39 |
| Tenericutes | 0.04 | 0.15 | 0.00 | 0.00 |
| d 42 | ||||
| Actinobacteria | 0.23 | 0.46 | 0.14 | 0.16 |
| Bacteroidetes | 2.12 | 0.85 | 0.56 | 0.12 |
| Epsilonbacteraeota | 0.26 | 0.19 | 0.05 | 0.20 |
| Firmicutes | 78.61 | 75.33 | 85.92 | 80.83 |
| Proteobacteria | 1.94 | 5.23 | 0.74 | 4.29 |
| Tenericutes | 0.06 | 0.08 | 0.03 | 0.00 |
Taxonomic diversity table shows the relative abundance (%) of taxa at the phylum level in each group. The relative abundance of each sample was calculated based on the amplicon sequence variants table rarefied to 10,891 and 29,818 reads per sample for d 8 and d 42, respectivelya.
Data represent the mean value of 1 bird/pen (8 birds/treatment).
Treatments include: negative control (NC): corn-soybean meal basal diet; positive control (PC): NC + 453 g virginiamycin (Stafac®20)/ton diet; probiotic (PROB): NC + 227 g B. licheniformis spores/ton diet; probiotic/prebiotic/essential oil supplement (PPEO): NC + 453 g of a supplement containing B. licheniformis spores, mannan-oligosaccharides, β-glucans (1,3 and 1,6), capsaicin and curcuma/ton diet.