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. 2020 Dec 9;10:21536. doi: 10.1038/s41598-020-78401-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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FLAT spectra are distinctive by bacterial species and similar to equivalent Microextraction spectra. (A) A heatmap demonstrating that FLAT produces spectra consistent with microextraction. (B) A heatmap demonstrating FLAT species and mcr-1 status differentiation. In both cases, similarity between two groups of spectra was computed via the mean cosine similarity across all pairs of spectra, and is visualized as a circle with a proportionally scaled diameter. A total of 149 FLAT spectra and 148 microextraction spectra were used in this comparison, from strains and samples as described in Supplementary Tables S1 and S2 online. CR: mcr-1 transformed; Ab: Acinetobacter baumannii; Ec: Escherichia coli; Kp: Klebsiella pneumoniae Pa: Pseudomonas aeruginosa; Mm: Morganella morganii; Sm: Serratia marcescens; Bc: Bacillus cereus; Bw: Bacillus mycoides (previously known as Bacillus weihenstephanensis³⁷); Sa: Staphylococcus aureus.