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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: J Immunol. 2020 Nov 11;205(12):3333–3347. doi: 10.4049/jimmunol.2000829

Figure 2: Kinetics of ear dermis, ear draining lymph nodes and splenic neutrophil recruitment following LdCen−/− intradermal infection.

Figure 2:

Mice were injected intradermally either with PBS or LdWT or LdCen−/− parasites for different time periods as indicated. Kinetics of neutrophil recruitment in ear dermis, ear draining lymph node (dLN) and spleen was analyzed by flow cytometry. Neutrophils were defined as a subpopulation of CD11b+myeloid cells having the following markers: Ly6CintLy6G+. Changes in the total number of neutrophils per (A) ear dermis (C) ear dLN and (E) spleen. Values shown are the mean numbers of cells per ear dermis/ear dLN / spleen ± standard deviations of results. 6 ears, ear dLN and spleen at each time point, pooled data from 3 independent experiments (n=6). (*P < 0.05, ** P < 0.005) (B) Changes in the subsets of parasitized (RFP+/ mCherry+) neutrophils in ear dermis at 6h post infection and (D) ear dLN at 48h post infection expressed as a percentage of the total RFP+/ mCherry+ neutrophils. The data presented are means ± SD of 3 independent experiments (n=6).