Skip to main content
. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: J Immunol. 2020 Nov 11;205(12):3333–3347. doi: 10.4049/jimmunol.2000829

Figure 3. Increased CXCL1/CXCL2 production by ear tissue resident macrophages following LdCen−/−intradermal infection.

Figure 3.

(A) Mice were either not infected (PBS injected) or infected intradermally in the ear pinna with LdWT or LdCen−/− parasites for 6h. Ear tissue resident macrophages were sort selected from different groups of mice (n=6) by gating live single cells for Cd11b+ F4/80+ Ly6G− Ly6C−MHCII−. The sorting strategy is displayed. (B) The absolute number of tissue resident macrophages obtained per ear dermis of the infected mice were calculated. The experiment was repeated 3 times with pooled digests from 6 ear dermis per experiment. Means and standard errors of the means for 6 mice in each group are shown. Data are representative of 3 independent experiments. (C, D) Changes in the expression of CXCL1 and CXCL2 mRNA in sort selected ear tissue resident macrophages were determined by qPCR as described in Material and Methods. Data are presented as fold change from uninfected naive mice. The data represent the mean values ± standard deviations of results from 3 independent experiments that all yielded similar results (n=6). *** P < 0.0005.