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. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: J Immunol. 2020 Nov 9;205(12):3383–3389. doi: 10.4049/jimmunol.2000686

Fig. 2. FLS from C3H/HeN mice infected with B. burgdorferi have a trained phenotype while FBs are tolerized.

Fig. 2

Hip synovium and ears/tails from naïve or eight weeks B. burgdorferi-infected C3H/HeN mice were collected and FLS (A) and FBs (B) were extracted. Cells were culture for three to five passages before being seeded on a plate. B. burgdorferi were then added at a MOI of 10 for 24 hours. Supernatants were collected and cytokines (IL-6, MIP1a, CXCL1 and TNF-a) expression was measured by ELISA. Bars represent the mean ± SEM of at least triplicates. Data are representative of at least two independent experiments conducted with three animals in each group. ***P < 0.001.