Skip to main content
. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: J Immunol. 2020 Nov 9;205(12):3383–3389. doi: 10.4049/jimmunol.2000686

Fig. 3. FLS from arthritis resistant C57BL/6 mice do not get trained during B. burgdorferi infection.

Fig. 3

Hip synovium from naïve or eight weeks B. burgdorferi-infected C57BL/6 mice were collected and FLS were extracted. Cells were culture for three to five passages before being seeded on a plate. B. burgdorferi were then added at a MOI of 10 for six or 24 hours. Supernatants and cells were collected and cytokines (IL-6, MIP1a, CXCL1 and TNF-a) expression was measured by ELISA (A) and qPCR (B). Bars represent the mean ± SEM of at least triplicates. Data are representative of two independent experiments conducted with three animals in each group. *P < 0.05; **P < 0.01.