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. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: J Immunol. 2020 Nov 9;205(12):3383–3389. doi: 10.4049/jimmunol.2000686

Fig. 5. The B. burgdorferi-induced training in FLS does not require the TLR2-MyD88 pathway.

Fig. 5

Hip synovium from WT C57BL/6 mice, tlr2 ko mice (A) or myd88 ko (B) mice were collected and FLS were extracted. Cells were culture for three to five passages before being seeded on a plate. B. burgdorferi were then added at a MOI of 10 for 24 hours (24h). Cells were washed with PBS and fresh B. burgdorferi were added to the cells at a MOI of 10 for another 24 hours (24h/24h). Supernatants were collected and cytokines (IL-6, MIP1a, CXCL1 and TNF-a) expression was measured by ELISA. Bars represent the mean ± SEM of at least triplicates. Data are representative of two independent experiments. *P < 0.05; ***P < 0.001.