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. 2020 Nov 25;11:577108. doi: 10.3389/fphar.2020.577108

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Copyright © 2020 Wang, Liang, Yao, Yang, Zhang, Liu, Li, Pei, Ma, Xie and Fang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EGF promotes PD-L1 expression in hepatocellular carcinoma cells (A and B) A significant increase in PD-L1 mRNA (A) and protein expression (B) was observed in Huh-7, HepG2, SNU-368 and SNU-739 cells after treated with EGF for indicated time. *p < 0.05, **p < 0.01, ***p < 0.001 compared to control group, one-way ANOVA, n = 5 independent experiments per group (C) The ELISA assay showed that the concentration of PD-L1 in the cell culture medium was increased after incubation with EGF for indicated time, *p < 0.05, **p < 0.01, ***p < 0.001 compared to control group, one-way ANOVA, n = 5 independent experiments per group.