FIGURE 3.

EGF stimulates the phosphorylation and nuclear translocation of PKM2 in SNU-368 cells (A) EGF induced an increase in the level of PKM2 Ser³⁷ phosphorylation in SNU-368 cells. **p < 0.01, ***p < 0.001, one-way ANOVA, n = 6 per group (B) Gefitinib attenuated EGF-induced the level of PKM2 Ser³⁷ phosphorylation in SNU-368 cells. **p < 0.01, one-way ANOVA, n = 5 per group (C) EGF-mediated PKM2 nuclear accmulation was reversed by Gefitinib in SNU-368 cells. Lamin B1 was used as internal control and β-actin was used as negative control. **p < 0.01, one-way ANOVA, n = 5 per group (D) Immunofluorescence staining showed Gefitinib attenuated EGF-induced PKM2 nuclear accmulation in SNU-368 cells.