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. 2020 Nov 25;11:577108. doi: 10.3389/fphar.2020.577108

FIGURE 4.

FIGURE 4

Phosphorylation of PKM2 at Ser37 participates in EGF‐induced PD‐L1 expression. (A,B) PKM2 shRNA blocked EGF‐induced PD‐L1 mRNA (A) and protein (B) expressions in SNU‐368 cells. At 24 h post‐transfection, the cells were incubated in the presence or absence of 100 ng/ml EGF for 12 h. **p < 0.01, ***p < 0.001, one‐way ANOVA, n = 4 independent experiments per group. (C,D) The expression of a phosphorylation‐mimic PKM2 S37D mutant induced a higher expression of PD‐L1 mRNA (C) and protein (D) compared with WT PKM2 or the S37A mutant in SNU‐368 cells. **p < 0.01, one-way ANOVA, n = 4 independent experiments per group. (E) ChIP analyses showed that the expression of a phosphorylation‐mimic PKM2 S37D mutant resulted in increased binding of PKM2 to PD‐L1 promoter in SNU-368 cells. *p < 0.05, **p < 0.01, one-way ANOVA, n = 5 independent experiments per group.