FIGURE 6.

DEN treatment induced a significant upregulation of phospho-EGFR, phospho-H3, and PKM2 nuclear accumulation in rat livers. (A) Top, representative photos of livers from normal and DEN-treated rats; bottom, representative images of H&E-stained livers. (B) The phosphorylational level of EGFR at Tyr¹⁰⁶⁸ was increased in the livers of DEN-treated rats. **p < 0.01, two-tailed unpaired t-test, n = 8 rats per group. (C) The expression of PKM2 nuclear protein was upregulated in the livers of DEN-treated rats. Lamin B1 was used as an internal control, and β-actin was used as a negative control. **p < 0.01, two-tailed unpaired t-test, n = 8 rats per group. (D) The phosphorylational level of H3-Thr¹¹ was increased in the livers of DEN-treated rats. **p < 0.01, two-tailed unpaired t-test, n = 8 rats per group. (E,F) The expression of PD-L1 mRNA (E) and protein (F) was increased in the livers of DEN-treated rats. **p < 0.01, ***p < 0.001, two-tailed unpaired t-test, n = 8 rats per group. (G) ChIP analyses showed that DEN administration resulted in enhanced H3-Thr¹¹ phosphorylation at the PD-L1 promoter in rats. **p < 0.01, two-tailed unpaired t-test, n = 8 rats per group.