Extended Data Figure 3.

a, UPKII^Cre; Ai9 bladder tissue fixed, frozen and mounted to show tdTomato (red) throughout the urothelium, labeled with DAPI (blue). Expression was evaluated in two mice. b, SNS^Cre: Ai9 bladder tissue fixed, frozen and mounted to show tdTomato (red) is not present. Expression was evaluated in two mice. Thin cryosections made neuronal endings difficult to visualize. Scale: 200 μm, applies to a and b. c, SNS^Cre: Ai9 DRG tissue showing tdTomato (red) in the majority of neurons, and d, a cell backlabled with CTB-Alexa 488 injected into bladder. e, merge of c and d, DAPI in blue. 9/9 backlabeled bladder cells analyzed from two mice were tdTomato positive. f, Quantification of freshly excised bladder weights from four UPKII^Cre; Piezo2^f/f knockout and wildtype littermates. Age-matched littermates were 10–11 months old, which could account for greater variability. g, Bladder weights from age-matched SNS^Cre;Piezo2^f/f knockout mice and wildtype littermates, 7–8 months old. Red lines indicate mean values.