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. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: J Immunol. 2020 Nov 13;205(12):3311–3318. doi: 10.4049/jimmunol.2000985

Figure 2.

Figure 2.

The PE and E+6.5 enhancer contain IgE-responsive elements. (A) Schematic diagrams showing enhancer reporter constructions. (B) Enhancer reporter analysis of PE enhancer transfected CFTL-15 cells that were not stimulated (UN) or stimulated with IgE receptor crosslinking (IgECL). (C) Enhancer reporter analysis of the E+8.9 enhancer. (D) Enhancer reporter analysis of the E+6.5 enhancer. (E) Enhancer reporter analysis of the E+2.5 enhancer. (F) Enhancer reporter analysis of the E+0.2 enhancer. (G) Enhancer reporter analysis of the E-0.1 enhancer. Mean ± SD were calculated from 3 transfectants in one experiment, representing two experiments with similar results. Statistical differences were analyzed by student’s t test. *: P<0.05, **: P<0.01, ***: P<0.001. MP, the minimal Il13 promoter.