Figure 4.

Combinatorial NFATC2, STAT5, GATA2, AP1 and RUNX1 binding sites at the PE enhancer are essential for the IgE receptor crosslinking-induced PE enhancer activity. (A) OmniATAC-seq tracks at the −300 to +200 region of the Il13 gene in BMMCs that were not stimulated (UN) or stimulated with IgE receptor crosslinking (IgECL). Lower panel represents enhancer reporter analysis of the P150 enhancer reporter transfected CFTL-15 cells that were not stimulated (UN) stimulated with IgE receptor crosslinking (IgECL). (B) Upper panel depicts schematic diagrams showing various mutations at the PE region. Mutated sequences are indicated inside the boxed. Lower panel, enhancer reporter analysis of transfected CFTL-15 cells that were not stimulated (UN) stimulated with IgE receptor crosslinking (IgECL).

Mean ± SD were calculated from 3 transfectants in one experiment, representing two experiments with similar results. Mut, mutant; Mut_NS, NFATC2 and STAT5 double mutants; Mut_GAR, GATA2, AP1 and RUNX1 triple mutants.