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. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: J Immunol. 2020 Nov 13;205(12):3311–3318. doi: 10.4049/jimmunol.2000985

Figure 5.

Figure 5.

The EGR2 binding site at the E+6.5 enhancer mediates the IgE receptor crosslinking-induced E+6.5 enhancer activity. (A) Top panel shows Omni-ATAC-Seq peaks at the E+6.5 enhancers in resting (UN) and stimulated BMMC with IgE receptor crosslinking (IgECL). Middle panel is the schematic diagram of enhancer deletional analysis. The dotted box highlights the enhancer segments that lost its responsiveness to IgE receptor crosslinking. Lower panel, enhancer reporter analysis of the fragment from position +7370 to +7570 of the E+6.5 enhancer. (B) Enhancer reporter analysis of the fragment from position +7470 to +7520 of the E+6.5 enhancer. (C) Mutational analysis of the fragment from position +7470 to +7520 of the E+6.5 enhancer. Mut_EFS, EGR2, FOX2 and STRE triple mutants. Mean ± SD were calculated from 3 transfectants in one experiment, representing two experiments with similar results.