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. Author manuscript; available in PMC: 2021 Jan 10.
Published in final edited form as: Mucosal Immunol. 2020 Jun 9;14(1):100–112. doi: 10.1038/s41385-020-0311-9

Fig. 5. Impact of RA and ligand binding ability on nuclear versus cytoplasmic RARα localization.

Fig. 5.

(a) Domains and the mutation site for G303E in the RARα protein. (b) Confocal images of FLAG-RARα expressed in CD4+ T cells. FLAG-RARα was expressed in CD4+ T cells by retroviral gene transfer. Naïve CD4+ T cells were activated overnight and transduced with recombinant retrovirus particles and further cultured in a Th17 cell cytokine condition for 16h without or with RA (10 nM). (c) Cytoplasmic localization of FLAG-RARα as a percentage of total cell signal was measured using ImageJ. *Significant differences (P values <0.05) from indicated groups by two-way ANOVA with Bonferroni (c).