Figure 2. GKN3 is expressed by SPEM cells in the murine gastric corpus.

(A) Representative RNAscope images of healthy antrum, healthy corpus, and atrophic corpus from TxA23 mice with chronic gastritis. Nuclei identified with DAPI (white). The identified transcripts were Gkn3 (cyan), Tff2 (yellow), and Muc6 (red). Fluorescent images are representative of 3–5 mice per group from 1–2 separate experiments. (B) Representative fluorescent images gastric corpus from healthy mice, mice with chronic autoimmune gastritis (“Autoimmune”) and mice with chronic Helicobacter pylori infection (“H. pylori”). Tissue is stained with anti-GIF (red), GSII (green), anti-TFF2 (magenta), and anti-GKN3 (yellow).