Skip to main content
. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: J Immunol. 2020 Nov 9;205(12):3247–3262. doi: 10.4049/jimmunol.2000748

Figure 3.

Figure 3.

Fgl2 regulates B cell CSR in vitro (a-c) Effect of Fgl2 on B cell CSR: In vitro class switching assay. Sorted total B cells (CD19+CD4−) nonimmunized wild-type, Fcgr2b−/− or Fcgr3−/− mice for 7 days were cultured in LPS + IL4 (IgG1 and IgE) or LPS + IFNG (IgG2b) for 4 days in the presence or absence of Fc block. Switched isotypes were detected by flow cytometry. Differences between two groups were compared Kruskal-Wallis tests followed by post hoc Dunn’s tests for multiple comparisons (n.s; * p <0.05, ** p<0.01 and *** p<0.001). All plots show the means ± SD with n = 3 in each group.