Figure 1.

Confocal Images and Multi-Signal Reconstructions of da Neurons

Class I wild-type (A–D), class IV wild-type (E–H), and class IV mutant Form3OE (I–L) neurons are analyzed in this study. Microtubule (A, E, and I) and F-actin (C, G, and K) signals from confocal image stacks are traced together to produce multi-signal reconstructions, allowing the arbor-wide quantification and graphic rendering of microtubule (B, F, J) and F-actin (D, H, and L) signal (heatmaps represent cytoskeletal quantity, calculated by multiplying the local intensity by the compartment thickness: see Transparent Methods for a detailed description of microtubule and F-actin quantification). Axons (identified with arrows in A, C, E, G, I, and K), only partially visible, are not traced and analyzed in this study. White scale bars (B, D, F, H, J, and L), 100 μm. Figure S1 shows magnified versions of the areas “demarcated” by the dotted lines (A–L). See also Figures S2 and S3.