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. 2020 Nov 26;23(12):101863. doi: 10.1016/j.isci.2020.101863

Figure 1.

Figure 1

Infarct Limiting Effect of Rapamycin (RAPA) in Diabetic Rabbits Following Ischemia/Reperfusion (I/R) Injury

(A) Experimental protocol for inducing T1D. Rabbits were treated with alloxan monohydrate and blood glucose levels were monitored for 4 weeks. Animals with consistent blood sugar of 220 mg/dL (blue dashed line) or above were considered diabetic. The average blood glucose level is denoted by red line.

(B) Experimental Protocol for I/R in conscious diabetic rabbits.

(C) Myocardial infarct size (% of risk area) and risk area (% of LV). Scale indicates 5 mm. n = 7/group; ∗p < 0.001 versus I/R and DM + I/R.

(D) Plasma levels of cardiac troponin I following I/R in controls (I/R), diabetics rabbits (DM + I/R), and diabetic rabbit treated with RAPA at the onset of reperfusion (DM + I/R + RAPA). n = 10; ∗p < 0.0001 versus I/R and DM + I/R + RAPA; αp<0.01 versus DM + I/R + RAPA.

(E) Oxidative stress was measured by lipid peroxidation assay for formation of malondialdehyde (MDA) in the hearts. n = 4; ∗p < 0.0001 versus control and DM; αp<0.0001 versus I/R; βp<0.0001 versus DM + I/R. Statistics: one-way ANOVA. Data are represented as mean ± SEM.