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. 2020 Nov 26;23(12):101863. doi: 10.1016/j.isci.2020.101863

Figure 3.

Figure 3

Rapamycin (RAPA) Reduces Myocardial Apoptosis Following I/R Injury in Diabetic Rabbits

(A) Apoptosis in the LV risk area was assessed by TUNEL assay. Representative pictures of TUNEL (Green), DAPI (Blue), and troponin (Red: Alexa 594) staining. Scale indicates 100 μm.

(B) Percentage of apoptotic nuclei to total nuclei in control, I/R, DM, DM + I/R, and DM + IR + RAPA groups. n = 6; ∗p < 0.0001 versus control and DM; αp<0.0001 versus others.

(C) Representative immunoblots showing expression of Bcl-2 and Bax and densitometry analysis of the ratio of Bcl-2 to GAPDH (n = 6; ∗p < 0.0001 versus others); Bax to GAPDH (n = 6; ∗p < 0.0001 versus control and DM; αp<0.0001 versus control; δp<0.01 versus control and DM); Bcl-2 to Bax (n = 6; ∗p < 0.05 versus control; αp<0.01 versus control, I/R and DM; δp<0.0001 versus others). Statistics: one-way ANOVA. Data are represented as mean ± SEM.