Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 26;23(12):101863. doi: 10.1016/j.isci.2020.101863

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Rapamycin Differentially Regulates mTORC1 and mTORC2 in Diabetic Rabbits Following I/R Injury

(A) Upper panel shows the representative immunoblots and lower panel shows densitometry analysis of the ratios of phosphorylation of S6 to total S6 (p-S6/S6; n = 6; ∗p < 0.0001 versus control, DM, DM + I/R + RAPA; ^αp<0.0001 versus I/R), phosphorylation of AKT to total AKT (p-AKT/AKT; n = 6; ∗p < 0.01 versus control and DM; ^αp<0.0001 versus control, DM and DM + I/R + RAPA), and total proteins to GAPDH (S6/GAPDH; n = 6; ∗p < 0.05 versus control, I/R and DM and AKT/GAPDH; n = 6; ∗p < 0.001 versus others).

(B) Real-time PCR analysis shows the levels of miR-302a (∗p < 0.05 versus control and DM; ^αp<0.001 versus others and ^βp<0.001 versus control and DM).

(C) Real-time PCR analysis shows the levels of PTEN mRNA (∗p < 0.05 versus control and DM; ^αp<0.001 versus others).

(D) Representative immunoblots (upper panel) and densitometry analysis (lower panel) of the expression of PTEN and GAPDH in hearts of control and diabetic rabbits before/after I/R and with/without RAPA treatment (n = 6; ∗p < 0.0001 versus others). Statistics: one-way ANOVA. Data are represented as mean ± SEM.