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. 2020 Nov 26;23(12):101863. doi: 10.1016/j.isci.2020.101863

Figure 7.

Figure 7

miR-302a Mimic Protects hiPSC-CMs Against Simulated Ischemia and Reoxygenation (SI/RO) Injury Under Normal (NG) and High Glucose (HG) Conditions

(A) Necrosis of hiPSC-CMs transfected with miR-302a mimic and following SI/RO injury under NG and HG conditions. n = 6; ∗p < 0.0001 versus others; αp<0.01 versus SI/RO (NG); βp<0.001 versus SI/RO (NG & HG).

(B) Apoptosis with representative pictures of TUNEL-DAPI staining in hiPSC-CMs transfected with miR-302a mimic and following SI/RO injury under NG and HG conditions. n = 6; ∗p < 0.0001 versus others; αp<0.01 versus SI/RO (NG); βp<0.001 versus SI/RO (NG & HG). Scale indicates 100 μm.

(C) Representative immunoblots showing the effect of miR-302a overexpression on expression of Bcl-2 and Bax in hiPSC-CMs following SI/RO under NG and HG conditions. Right panel shows the densitometry analysis of Bcl-2 to GAPDH (n = 3; ∗p < 0.005 versus control (NG & HG)); αp<0.005 versus control & SI/RO (NG & HG)); Bax to GAPDH (n = 3; ∗p < 0.05 versus control (NG); αp<0.05 versus control (HG) & SI/RO (NG & HG)) and Bcl-2 to Bax ratio (n = 3; ∗p < 0.05 versus control (NG & HG); αp<0.005 versus SI/RO (NG & HG). Statistics: one-way ANOVA. Data are represented as mean ± SEM.