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. 2020 Nov 26;23(12):101863. doi: 10.1016/j.isci.2020.101863

Figure 8.

Figure 8

miR-302a Regulates PTEN-AKT-mTORC2 Signaling in hiPSC-CMs Under Normal (NG) and High Glucose (HG) Conditions

(A) Representative Immunoblots showing the effect of overexpression of miR-302a on expression of phosphorylation of S6 in hiPSC-CMs following SI/RO under NG and HG conditions. Bottom panel presents the densitometry analysis (n = 3) of pS6/S6; ∗p < 0.005 versus control & miR-302 mimic (NG & HG) and S6/GAPDH; ∗p < 0.05 versus control (NG).

(B) Representative immunoblots showing the phosphorylation level of AKT in hiPSC-CMs transfected with miR-302a mimic following SI/RO under NG and HG conditions. Bottom panel presents the densitometry analysis (n = 3) of pAKT/AKT; ∗p < 0.005 versus miR-302a mimic + SI/RO (NG & HG); αp<0.05 versus control (HG) and AKT/GAPDH.

(C) PTEN expression in hiPSC-CMs transfected with miR-302a mimic following SI/RO under NG and HG conditions. Lower left panel shows densitometry analysis of PTEN with GAPDH as loading control (n = 3; ∗p < 0.005 versus others). Lower right panel presents real-time PCR analysis of PTEN mRNA in hiPSC-CMs transfected with miR-302a mimic following SI/RO under NG and HG conditions. n = 3; ∗<0.0001 versus others. Statistics: one-way ANOVA. Data are represented as mean ± SEM.