FIGURE 3.

FcγR3A-mediated responses. (A) Jurkat NFAT-luciferase cells expressing individual allotypes of human and rhesus macaque FcγR3A were incubated overnight with Raji cells at a 2:1 E:T ratio in the presence of serial dilutions of anti-CD20 antibodies with Fc domains corresponding to each of the four subclasses of human and rhesus macaque IgG and variants of IgG1 that enhance binding to FcγR2A (G236A) or FcγR3A (DEL). Curves were fit to the data using GraphPad Prism software. Error bars indicate SD of the mean for triplicate measurements at each antibody concentration. (B) Antibody concentrations for half-maximal responses (EC₅₀) were calculated from dose response curves. EC₅₀ values that could not be determined within the range of antibody concentrations tested are not shown. Error bars indicate SD of the mean for at least three independent experiments.